THE FUNCTIONING FEATURES OF PROOXIDANT-ANTIOXIDANT SYSTEM AND IMMUNE PROTECTION DURING DENTAL CARIES TREATMENT IN CHILDREN WITH NEUROPSYCHIATRIC DISORDERS

Aim. To study the functioning of the prooxidant-antioxidant system and immune defense in the treatment of dental caries in children with psychoneurological disorders in different age groups.

Materials and methods. Biological material for the study was oral fluid of patients with tooth decay, with neuropsychiatric disorders (n=84 children) and without comorbid pathology (n=47 children), the control group was 45 people. An evaluation of the severity of the disturbances in the prooxidant-antioxidant system in the oral fluid was carried out by determining the intensity of chemiluminescence in the maximum and the area of the outbreak, as well as the overall antioxidant activity characterizing the state of the antioxidant system. Among the indices of the enzyme link of antioxidant protection, the activity of superoxide dismutase, catalase was determined, and in addition, the integral index of the functioning of antiradical protection enzymes was calculated. Investigation of the state of local immune defense was carried out by determining the content of proinflammatory and pro-inflammatory interleukins, and also assessing the level of lactoferrin.

 Conclusion. The functioning of the prooxidant-antioxidant system and the regulation of immune defense in children with psychoneurological disorders in different age groups have a number of characteristics with which the clinical indices correlate. The use of complex dental care ("Multistep" regulations) allows more complete rehabilitation of patients and achieving significantly better results in normalizing the biochemical state of the oral fluid.

1. Diab H.A., Salameh Z., Hamadeh G.N. et al. Oral health status of institutionalized individuals with intellectual disabilities in lebanon. Journal of Oral & Maxillofacial Research. 2017; 8(1): e4. DOI: 10.5037/jomr.2017.8104.

2. Morgan J.P., Minihan P.M., Stark P.C. et al. The oral health status of 4,732 adults with intellectual and developmental disabilities. J. Am. Dent. Assoc. 2012; 143(8): 838-846. DOI: 10.14219/ jada.archive.2012.0288.

3. Jaber M.A. Dental caries experience, oral health status and treatment needs of dental patients with autism. J. Appl. Oral. Sci. 2011; 19(3): 212-217. DOI: 10.1590/S1678-77572011000300006.

4. Oda Y., Hayashi F., Okada M. Longitudinal study of dental caries incidence associated with Streptococcus mutans and Streptococcus sobrinus in patients with intellectual disabilities. BMC Oral Health. 2015; 15(1): 102. DOI: 10.1186/s12903-015-0087-6.

5. Bradshaw D.J., Lynch R.J.M. Diet and the microbial aetiology of dental caries: new paradigms. Int. Dent. J. 2013; 63(2): 64-72. DOI: 10.1111/idj.12082. [Bradshaw D.J., Lynch R.J.M. Diet and the microbial aetiology of dental caries: new paradigms. Int. Dent. J. 2013; 63(2): 64-72. DOI: 10.1111/idj.12082].

6. Litvinova M.G., Basov A.A., Bykov I.M. Indicators of free radical oxidation in blood and oral liquid of patients with coronary heart disease and type 2 diabetes mellitus. Kubanskij nauchnyj medicinskij vestnik. 2012; 3: 94-98. (In Russ., English abstract).

7. [Huang Y., Zhu M., Li Z., Sa R., Chu Q., Zhang Q., Zhang H., Yin H. Mass spectrometry-based metabolomic profiling identifies alterations in salivary redox status and fatty acid metabolism in response to inflammation and oxidative stress in periodontal disease. Free Radical Biology and Medicine. 2014; 70: 223-232. DOI: 10.1016/j.freeradbiomed.2014.02.024.

8. Pavlyuchenko I.I., Dynko Yu.V., Basov A.A., Fedosov S.R. Integral indicators of endogenous intoxication and oxidative stress in patients with renal insufficiency. Nephrology and Dialysis. 2003; 5(S1): 28-32. (In Russ).

9. Tothova L., Kamodyova N., Cervenka T., Celec P. Salivary markers of oxidative stress in oral diseases. Frontiers in Cellular An Infection Microbiology. 2015; 5: 1-40. DOI: 10.3389/fcimb.2015.00073. [

10. Trivedi S., Lal N., Mahdi A.A. et al. Association of salivary lipid peroxidation levels, antioxidant enzymes, and chronic periodontitis. Int. J. Periodontics Restorative Dent. 2015; 35: e14-e19. DOI: 10.11607/prd.2079.

11. Pavlyuchenko I.I., Basov A.A., Orlova S.V., Bykov I.M. Change in the activity of anti-radical defense enzymes as a prognostic criterion for the development and progression of diabetes mellitus. International Journal on Immunorehabilitation. 2004; 6(1): 14-19. (In Russ., English abstract).

12. Kamodyova N., Tothova L., Celec P. Salivary markers of oxidative stress and antioxidant status: influence of external factors. Disease Markers. 2013; 34(5): 313-321. DOI: 10.3233/DMA130975.

13. Bykov M.I., Basov A.A. Change of parameters in prooxidant-antioxidantbile system in patients with the obstruction of bile-excreting ducts. Medical news of North Caucasus. 2015; 10(2): 131-135. DOI: http://dx.doi.org/10.14300/mnnc.2015.10029.

14. Silva P.V.D., Troiano J.A., Nakamune A.C.M.S. et al. Increased activity of the antioxidants systems modulate the oxidative stress in saliva of toddlers with early childhood caries. Archives of Oral Biology. 2016; 70(1): 62-66. DOI: 10.1016/j.archoralbio.2016.06.003.

15. Pavlyuchenko I.I., Dynko Yu.V., Basov A.A. Indicators of endogenous intoxication and oxidative stress in patients with diabetes mellitus against the background of decompensated ketoacidosis. Vestnik intensivnoy terapii. 2004; 5: 116-120. (In Russ., English abstract).

16. Basov A.A., Akopova V.A., Bykov I.M. Changing the parameters of prooxidant-antioxidant system in blood and oral fluid of patients with ischemic heart disease and type 2 diabetes mellitus. International Journal on Immunorehabilitation. 2013; 15(2): 84-86.

17. Pfaffe T., Cooper-White J., Beyerlein P. et al. Diagnostic potential of saliva: Current state and future applications. Clinical Chemistry. 2013; 57(5): 675-687. DOI: 10.1373/clinchem.2010.153767.

18. Dzhimak S.S., Baryshev M.G., Basov A.A. Content of deuterium in biological fluids and organs: influence of deuterium depleted water on D/H gradient and the process of adaptation. Doklady Biochemistry and Biophysics. 2015; 465(1): 370-373. DOI: 10.1134/S1607672915060071.

19. Zhang A., Sun H., Wang X. Saliva metabolomics opens door to biomarker discovery, disease diagnosis, and treatment. Applied Biochemistry and Biotechnology. 2012; 168(6): 1718-1727. DOI: 10.1007/s12010-012-9891-5.

20. Tiwari M. Science behind human saliva. J. Nat. Sc. Biol. Med. 2011; 2: 53-58. DOI: 10.4103/0976-9668.82322.

21. Bykova N., Basov A., Melkonyan K. et al. Non-invasive monitoring for local immune and antioxidant resistance in patients with ischemic heart disease and type 2 diabetes. Medical news of North Caucasus. 2016; 11(2). 147-149. DOI: http://dx.doi.org/10.14300/mnnc.2016.11021. [

22. Villa-Correa Y.A., Isaza-Guzmán D.M., Tobon-Arroyave S.I. Prognostic value of 8-hydroxy-2-deoxyguanosine and human neutrophil elastase/α1-proteinase inhibitor complex as salivary biomarkers of oxidative stress in chronic periodontitis. Journal of Periodontology. 2015; 86(11): 1260-1267. DOI: https://doi.org/10.1902/jop.2015.150293.

23. Pavlyuchenko I.I., Bykov M.I., Fedosov S.R. Basov A.A., Bykov I.M, Morgoev А.E., Gaivoronskaya T.V. Complex evaluation of status of pro-antioxidants system in various biological media in surgical patients with purulent septic complications. Advances in current natural sciences. 2006; 6: 82-83.(In Russ.).

24. Pavlyuchenko I.I., Basov A.A., Fedosov S.R. System of laboratory diagnostics of oxidative stress. Patent RUS 54787. 19.01.2006. 2 p. (In Russ.).

25. Pavlyuchenko I.I., Fedosov S.R., Basov A.A. Program for recording signals of chemiluminotester LT-1. Certificate of registration of the computer program RUS 2006611562. 16.03.2006. (In Russ.).

26. Kostyuk V.A., Potapovich A.I., Kovaleva Zn.V. A simple, sensitive assay for determination of superoxide dismutase activity based on reaction of quercetin oxidation. Problems of Medical Chemistry. 1990; 2: 88-91. (In Russ., English abstract).

27. Bykov I.M., Basov A.A., Malyshko V.V., Dzhimak S.S., Fedosov S.R., Moiseev A.V. Dynamics of the pro-oxidant/antioxidant system parameters in wound discharge and plasma in experimental purulent wound during its technological liquid phase treatment. Bulletin of Experimental Biology and Medicine. 2017; 163(2): 237-241. DOI: 10.1007/s10517-017-3781-3.

28. Karpishchenko A.I. Medical and laboratory technology. Reference book. SPb.: Intermedika; 2002; 2: 600 p. (In Russ.).

29. Basov A.A., Pavlyuchenko I.I., Bykov I.M., Fedosov S.R., Gubareva E.A. Method for diagnosing metabolic disorders in the body under oxidative stress. Patent RUS 2436101. 25.06.2010. 20 с. (In Russ.).

30. Yashin A.Y. A flow-injection system with amperometric detection for selective determination of antioxidants in foodstuffs and drinks. Russ. J. General Chem. 2008; 78(12): 2566-2571. DOI: 10.1134/S1070363208120360.