Study of Behavioural Activity and Cognitive Impairment in Rats in the Simulation of Pneumococcal Meningitis
https://doi.org/10.25207/1608-6228-2019-26-6-80-92
Abstract
Aim. To study neurological disorders in rats when modeling purulent bacterial meningitis (PBM) on the example of pneumococcal meningitis under experimental conditions.
Materials and methods. Pneumococcal meningitis was modeled by injecting a suspension containing Streptococcus pneumoniae at a concentration of 5×109 CFU/ml into the subarachnoid space. The degree of neurological disorders was determined by clinical assessment of rat health status, the degree of neurological defi cit, specifi c strength, “Infrared activity monitor”, “Object recognition task” tests.
Results. When modeling pneumococcal meningitis in rats, the sum of points of clinical assessment of their health in the 1st day after modeling the pathology is 34.2 % less than the initial, on the 5th — less than 3.4 %, on the 8th day returns to the primary indicator, 5 points. The maximum degree of severity of neurological defi cit was observed in the fi rst four days after meningitis modeling; it was in the 1st day 34 % less than the same indicator in the group of intact animals, on the 2nd day 32.7 % less, on the 3rd day — 30.7 % and on the 4th day — 30.1 %. In the meningitis group on day 10, the distribution of neurological defi cit by severity was as follows: mild — 32 %, medium — 20 %, severe — 16 %, without residual neurological defi cit — 32 %. The specifi c strength of rats in the group of meningitis on the 1st, 5th and 10th day after the development of the pathology was 48.7, 64, and 67.4 % of the baseline specifi c strength. In relation to intact animals, the index of recognition of short-term memory in rats of the meningitis group is 2.3 times greater, and the index of recognition of long-term memory is 1.7 times greater.
Conclusion. The dynamics of neurological disorders in rats in the simulation of PBM can be determined using the methods of assessing behavioural and cognitive status. In rats, when modeling pneumococcal meningitis, neurological disorders are maximally expressed on the 1st day of the disease. Then there is a distinct positive dynamic up to 5 days of the disease. From 6 to 10 days positive dynamics is present, but insignifi cant.
About the Authors
Alina A. Agarkova
Belgorod State National Research University;
Infectious Clinical Hospital named after E. N. Pavlovsky
Russian Federation
Russian Federation
Alina A. Agarkova - Postgraduate Researcher, Department of Pharmacology and Clinical Pharmacology; Infectious Disease Specialist, Infectious Disease Department No. 2
85, Pobedy str., Belgorod, 308015;
122 Sadovaya str., Belgorod, 308023
Mikhail V. Pokrovsky
Belgorod State National Research University
Russian Federation
Russian Federation
Mikhail V. Pokrovsky — Dr. Sci. (Med.), Prof., Head of the Department of Pharmacology and Clinical Pharmacology
85, Pobedy str., Belgorod, 308015
Pavel D. Kolesnichenko
Belgorod State National Research University
Russian Federation
Russian Federation
Pavel D. Kolesnichenko — Cand. Sci. (Med.), Ass. Prof., Department of Pharmacology and Clinical Pharmacology
85, Pobedy str., Belgorod, 308015
References
1. Martynova A.V., Turkutyukov V.B. Epidemiological analysis of the incidence of invasive and non-invasive pneumococcal infections in different population groups. Vestnik Rossiiskoi Akademii Meditsinskikh Nauk. 2007; 9: 12–16 (In Russ., English abstract).
2. Platonov A.E., Nikolaev M.K. Purulent meningitis morbidity in children under 5 years of age in the Regions of Russia. Epidemiologiya i Infektsionnye Bolezni. 2007; 3: 10–18 (In Russ., English abstract).
3. Thabet F., Tilouche S., Tabarki B., Amri F., Guediche M.N., Sfar M.T., Harbi A., Yacoub M., Essoussi A.S. Pneumococcal meningitis mortality in children. Prognostic factors in a series of 73 cases. Arch Pediat. 2007; 14(4): 334–337. DOI: 10.1016/j.arcped.2006.11.012
4. Scheld W.M., Koedel U., Nathan B., Pfi ster H.W. Pathophysiology of bacterial meningitis: mechanisms of neuronal injury. J. Infect. Dis. 2002; 186(Suppl 2): S225–233. DOI: 10.1086/344939
5. Vengerov Yu.Ya., Nagibina M.V., Mikhalinova E.P., Razdobarina S.E., Molotilova T.N., Parkhomenko Yu.G., Mozgaleva N.V., Smirnova T.Yu., Svistunova T.S. Pneumococcal meningitis: the problem of high mortality. Lechashchii Vrach. 2013; 5: 14 (In Russ.).
6. Pozdeev O.K. Medical microbiology. Moscow: Geotar-Med; 2004. 768 р. (In Russ.).
7. Kaplan S.L. Management of pneumococcal meningitis. Pediatr. Infect. Dis. J. 2002; 6(21): 589–591. DOI: 10.1097/00006454-200206000-00034
8. Shishov A.S., Grigorevskaya U.B., Gur’yanov A.V., Danilova L.V., Savrasova N.M., Solov’eva L.Ya., Kupriyanova L.V., Zaikin V.L, Petryaev V.G., Rudometov Yu.P., Rusanova S.A., Shuba G.A., Arsen’eva T.V., Kelli E.I. Some clinical feature sofbacterial infections with a syndrome of purulent meningitis. Zhurnal Nevrologii i Psikhiatriiim. S.S. Korsakova. 2011; 4(111): 90–95 (In Russ., English abstract).
9. Chavez-Bueno S., McCracken G.H. Bacterial meningitis in children. Pediatr. Clin. North. Am. 2005; 52(3): 795–810. DOI: 10.1016/j.pcl.2005.02.011
10. Agarkova A.A., Pokrovskii M.V., Korokina L.V., Zemlyanskii O.A. Diagnosis and current treatment of pneumococcal meningitis. Klinicheskaya Farmakologiyai Terapiya. 2019; 28(2): 84–92 (In Russ., English abstract). DOI: 10.32756/0869-5490-2019-2-84-92
11. Koedel U., Klein M., Pfi ster H.W. New understandings on the pathophysiology of bacterial meningitis. Curr. Opin. Infect. Dis. 2010; 23(3): 217–223. DOI: 10.1097/QCO.0b013e328337f49e
12. Barichello T., Savi G.D., Silva G.Z., Generoso J.S., Bellettini G., Vuolo F., Petronilho F., Feier G., Comim C., Quevedo J., Dal-Pizzol F. Antibiotic therapy prevents, in part, the oxidative stress in the rat brain after meningitis induced by Streptococcus pneumoniae. Neurosci. Lett. 2010; 478(2): 93–96. DOI: 10.1016/j.neulet.2010.04.072
13. Martynova O.V., Zhilinkova L.A., Gureev V.V., Martynov M.A., Beskhmelnitsyna E.A., Kostina D.A., Anciferov O.V., Shkileva I.Y. Research of behavioural reactions when modelling the total ischemia of the brain. Kuban Scientifi c Medical Bulletin. 2015; 6: 77–82 (In Russ., English abstract). DOI: 10.25207/1608-6228-2015-6-77-82
14. Barichello T., Simões L.R., Generoso J.S., Sangiogo G., Danielski L.G., Florentino D., Dominguini D., Comim C., Petronilho F., Quevedo J. Erythropoietin prevents cognitive impairment and oxidative parameters in Wistar rats subjected to pneumococcal meningitis. Transl. Res.2014; 163(5): 503–513. DOI: 10.1016/j.trsl.2013.12.008
15. Barrichello. T., Generoso J.S., Michelin C.M., Shoes L.R., Elias S.G., Volvo F., Comim C., Dal-Pizzol F., Quevedo J. Inhibition of matrix metalloproteinases-2 and -9 prevents cognitive impairment caused by pneumococcal meningitis in Wistar rats. Exp. Biol. Med. (Maywood). 2014; 239(2): 225–231. DOI: 10.1177 / 1535370213508354
Review
For citations:
Agarkova A.A., Pokrovsky M.V., Kolesnichenko P.D. Study of Behavioural Activity and Cognitive Impairment in Rats in the Simulation of Pneumococcal Meningitis. Kuban Scientific Medical Bulletin. 2019;26(6):80-92. (In Russ.) https://doi.org/10.25207/1608-6228-2019-26-6-80-92
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