Experimental Evaluation of Cryoprotector Systemic Effects
https://doi.org/10.25207/1608-6228-2019-26-6-117-126
Abstract
Aim. Experimental evaluation of the effect of some cryoprotectors on crystallogenic properties of mice blood serum at intraperitoneal administration.
Material and methods. The experiment was performed on 40 adult white line mice divided into 4 equal groups. Animals from the control (fi rst) group received a single intraperitoneal injection of saline (0.5 ml agent/animal), animals of the second-fourth groups — 3 % solution of glycerin, dimethyl sulphoxide and trombocryodmac (N, N-dimethylacetamide), respectively, in the same quantity.
Results. It is shown that single intraperitoneal introduction of cryoprotectants (solutions of glycerin, dimethyl sulphoxide and trombocryodmac) has a systemic effect on the mouse organism, which is manifested through the changes of crystallogenic properties of blood serum. 3% solution of glycerin, dimethyl sulphoxide and trombocryodmac have multidirectional infl uence on crystallogenic properties of mouse blood serum. The most pronounced physiological effect was established for dimethyl sulphoxide.
Conclusion. Our studies demonstrate the presence of systemic effects in cryopreservative substances. It was confi rmed by the changes of crystallogenic properties of mice blood serum under intraperitoneal administration of cryopreservatives.
About the Authors
Andrei K. Martusevich
Privolzhsky Research Medical University;
Kirov State Medical University
Russian Federation
Russian Federation
Andrey K. Martusevich — Dr. Sci. (Biol.), Leading Researcher, Head of the Laboratory of Medical Biophysics; Head of the Laboratory of Biocrystallomics and Free Radical Medicine
10/1 Minin sq., Nizhny Novgorod, 603000
112 K. Marx str., Kirov, 610998
Andrei A. Kostyaev
Kirov Research Institute of Hematology and Blood Transfusion under the Federal Medical Biological Agency
Russian Federation
Russian Federation
Andrei A. Kostyaev — Dr. Sci. (Med.), Ass. Prof., Senior Researcher, Laboratory of Cell Technologies
72 Krasnoarmeyskaya str., Kirov, 610021
Lida K. Kovaleva
Kuban State Medical University
Russian Federation
Russian Federation
Lida K. Kovaleva — Cand. Sci. (Med.), Research Assistant, Department of Histology with Embryology
4 Mitrofanа Sedina str., Krasnodar, 350063
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Review
For citations:
Martusevich A.K., Kostyaev A.A., Kovaleva L.K. Experimental Evaluation of Cryoprotector Systemic Effects. Kuban Scientific Medical Bulletin. 2019;26(6):117-126. (In Russ.) https://doi.org/10.25207/1608-6228-2019-26-6-117-126
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