Visualization of induction frog's sciatic nerve in a high-frequency electric field

Abstract

In order to visualize distribution of induction frog's sciatic nerve in a high electric field experiments were performed on 90 frogs Rana temporaria. In immobilized frogs were isolated sciatic nerve and placed it on the scanner camera gas discharge visualization install KELSEY, which creates an electric field high-frequency (1024 Hz). The 60-second video movie (frequency-lapse shooting 1000 frames per second) was shooted by scanner with a highly sensitive video cameras. The regional luminescence - effect Kirlian was observed in a frog's sciatic nerve in high-frequency electric field out of irritation. 4 hearth inner glows were appeared during nerve stimulation by single impulses. They disappeared after finishing the stimulation. This fact indicates that the inside of the nerve centers of luminescence reflects the induction of nerve fibers. The rate of emission of a luminescence corresponded to the speed of distribution induction in the nerve in m/s: (1 hearth) Aa = 46,2 ± 0,4; (center 2) Aß = 23,4 ± 0,5; (hearth 3) AY = 15,2 ± 0,4; (hearth 4) B = 10,0 ± 0,6. The centers of luminescence and their projections vary in size. Area of centers of luminescence in mm2 1 center - 8,0±0,4; 2 center - 4,3±0,4; 3 center - 3,2±0,2; 4 center - 2,4±0,4. The largest area of the centers of luminescence, moving to the muscle, has a center corresponding to the speed fiber Aa, then AY and Aß. The list area - center luminescence fibers of group B. Thus the visualization indicates the spread of induction in the frog's sciatic nerve.