Morphometric characteristic of nuclear junction zone myometrium cells in adenomyosis

Abstract

A morphometric study of cell nuclei intact junctional zone and in patients with adenomyosis. The material for the study is based on samples amputated during queens operations (47 cases), and autopsy material from apparently healthy women (6 cases). Wiring and sealing in paraffin. Measuring the optical density was nuclei (Feulgen reaction). The diameters of the cores were used to calculate the index of nuclear symmetry. We measure the amount of amorphous and fibrous components, as well as the total amount of hypochromic and hyperchromic nuclei. It was found that in patients with adenomyosis in the area junctional zone of endometrial stromal cell nuclei are characterized by an increase in the coefficient of ellipticity and absorbance. The nuclei of smooth muscle cells show pleomorphism and increase the index of the nuclear symmetry. The formed foci of adenomyosis identified unidirectional changes. It is possible that the nuclear symmetry index and the index of the optical density of the nuclei can be interpreted as molecular predictors of oncogenic transformation.