EFFECTS OF SODIUM HYPOCHLORITE ON THE PROTOSCOLICES OF ECHINOCOCCUS GRANULOSUS AND MEMBRANES OF HYDATID CYSTS

Aim. To assess sodium hypochlorite water solution anti-scolex activity against Echinococcus granulosus protoscolexies and effect on secondary echinococcus cysts envelopes.

Material and methods. We preformed two in vitro trials of the sodium hypochlorite water solution 0.06%, 0.12%, 0.25%, 0.5%, 1%, 2% and 4%. Sodium hypochlorite water solution anti-scolex activity versus exposure and the concentration of the drug was assessed in the first trial. Two drops of sodium hypochlorite water solution of various concentrations were added to the drop of the echinococcal fluid with living protoscalexes. The morphological changes of the parasite were investigate with a light microscope with a 100X magnification. The death of protoscolexes occurred within the first minute after the addition of sodium hypochlorite water solution, irrespective of solution concentration. The effect of sodium hypochlorite water solution on the secondary Echinococcus granulosus cysts envelopes was evaluated in the second trial. Envelopes fragments of the secondary echinococcal cysts were placed in tubes with sodium hypochlorite water solution in different concentrations. The changes were evaluated macroscopically. The envelopes of the secondary echinococcal cysts were completely dissolved in all tubes with sodium hypochlorite water solution.

Results. The experimental data have showed that sodium hypochlorite water solution is an effective anti-scolex medication enabling completely dissolution of the secondary echinococcal cysts envelops.

Conclusions. Considering that sodium hypochlorite is thoroughly investigated and safe for human body, sodium hypochlorite could be “ultimate” anti-scolex medication, enabling extension of indications and ad value for less invasive percutaneous interventions under radiation control in echinococcosis.

1. Agaev R.M. Diagnosis and surgical treatment of liver echinococcosis with biliary tract affection // Surgery. – 2002. №9. – Р. 58 – 63.

2. Liver echinococcal cysts puncture treatment method under the ultrasound control. A.V. Gavrilin, G.I. Kuntsevich, V.A. Vishnevsky, R.Z. Ikramov, Т.V. Zhurenkova, E.A. Burtseva, Т.V. Savvina, V.A. Agafonov // Surgery. – 2002. – No. 8. – Р. 39–46.

3. Chernyshev N.V. Surgery of liver echinococcosis / N.V. Chernyshev, S.A. Ivanov. – Samara: "Samarablankizdat", 2005. – Р. 196.

4. Akhan O. Percutaneous treatment of liver hydatid cysts / O Akhan, M.N. Ozmen // Eur J Radiol. – 1999. – Vol. 32. – P. 76-85.

5. Percutaneous Drainage Compared with Surgery for Hepatic Hydatid Cysts / M.S. Khuroo, N.A. Wani, G. Javid, B.A. Khan, G.N. Yattoo, A.H. Shah, S.G. Jeelani // New England Journal of Medicine. – 1997. – Vol. 337. – № 13. – P. 881–887.

6. Minimally invasive treatment of liver echinococcosis. R.M. Ahmedov, I.A. Mirkhodzhaev, U.B. Sharipov, B.Z. Khamdamov, B.B. Muazzamov // Annals of surgical hepatology. – 2010. – V. 15, No.3. – P. 99 – 104.

7. Treatment of hydatid echinococcosis. / S.A. Dadvani, S.A. Shkrob, A.N. Lotov, G.H. Musaev // Surgery. – 2000. – No. 8. – P. 27 – 32.

8. Liver echinococcosis surgical treatment methods evolution / Y.L. Shevchenko, S.S. Harnas., A.V. Samokhvalov, A.N. Lotov // Surgery. – 2004. – No. 7. – P. 49 – 55.

9. Vetshev P.S. Echinococcosis: the state of the problem / P.S. Vetshev, H.H. Musaev // Clinical perspectives of gastroenterology, hepatology. – 2005. – № 4. – P. 7 – 12.

10. Lotov A. N. Echinococcosis: Diagnosis and modern treatment methods / A.N. Lotov, A.V. Chzhao, N.R. Chermaya // Transplantology. – 2010. – No.2. – P.18 – 26.

11. Chernyshev V.N. Liver hydatidic echinococcosis treatment. / V.N. Chernyshev, K.A. Panfilov, V.E. Bogdanov // Surgery. – 2005. – No. 9. – P. 39 – 44.

12. Percutaneous Treatment of Hydatid Liver Cysts in Children as a Primary Treatment: Long-term Results / А.Y. Goktay, M. Secil, A. Gulcu, M. Hosgor, I. Karaca, M. Olguner, F.M. Akgur, O. Dicle // Journal of Vascular and Interventional Radiology. – 2005. – Vol. 16. – P. 831 – 839.

13. Helvig E. Therapeutic dentistry / E. Helvig, J. Klimek, T. Attin. – Lvov: "GalDent", 1999. – 409 p.

14. Fouad A.F. Endodontic Microbiology. – Wiley-Blackwell, 2009. – 368 р.

15. Burbello A.T. Modern medicines: Clinical and pharmacological reference book for the practicioner / A.T. Burbello, A.V. Shabrov. – Olma Media Group, 2007. – 396 p.

16. Clinical evaluation of the ocular safety of Amukine 0.06% solution for local application versus povidone iodine (Betadine) 5% solution for ocular irrigation) in preoperative antisepsis / F. Auclin, P. Rat, E. Tuil, C. Boureau-Andrieux, C. Morel, О. Laplace, С. Cambourieu, S. Limon, JP. Nordmann, L. Laroche, С. Baudouin // J Fr Ophtalmol. – 2002. – № 25. – P. 120 – 125.

17. Weisblatt J. Sodium Hypochlorite // Chemical Compounds / Project editor Charles B. Montney. – Thomson Gale. – 2006. – P. 759 – 763.

18. Fletcher J. Why life’s a bleach (The Sodium Hypochlorite Story) / J. Fletcher, D. Ciancon // Environmental Science and Engineering Magazine – May, 1996. – P. 27 – 28