ANALYSIS OF THE ANNEXIN V APOPTOSIS MARKER LEVEL AND DENTAL STATUS IN PATIENTS WITH CHRONIC GENERALIZED PERODONTITIS AND BRONCHOECTATIC DISEASE

Aim: Our aim was to study and analyze the level of the annexin V apoptosis marker in the oral fluid in patients with generalized periodontitis without general somatic pathology and against the background of bronchiectasis.

Materials and methods. 40 patients diagnosed with generalized periodontitis without somatic pathology and 50 patients diagnosed with generalized periodontitis and bronchiectasis were examined. The control group included 40 somatically healthy individuals with intact periodont. Indices characterizing the state of periodontal tissues, such as PMA, PI, Muhlemann, OHI-s were determined for all the examined persons. The level of the annexin V apoptosis marker was determined in the oral fluid by enzyme immunoassay.

Results. It was established that the value of the indices of periodontal tissues (PMA, PI, Muhlemann, OHI-s) was statistically signifi cantly higher in the group of patients with generalized periodontitis against the background of bronchiectasis both in comparison with the control group and with the group of patients without general somatic pathology. The level of the annexin V apoptosis marker was also statistically signifi cantly higher in patients with generalized periodontitis against the background of bronchiectasis as compared with the control group and the group of patients without general somatic pathology (p<0.001). According to the correlation analysis, statistically signifi cant relationships of the moderate strength between the level of annexin V and the value of the indices of periodontal tissues were found both in the group of patients without general somatic pathology and in the group of patients with generalized periodontitis against the background of bronchiectasis. At the same time, the strength of these relationships in patients with generalized periodontitis against the background of bronchiectasis was greater as compared to the group of patients without general somatic pathology.

Conclusion. The level of the annexin V apoptosis marker in the oral fluid of patients with generalized periodontitis against the background of bronchiectasis is statistically signifi cantly higher (p<0.001) than in patients with generalized periodontitis without general somatic pathology. The results of the correlation analysis indicate the presence of relationships between the level of annexin V and the value of periodontal tissue condition indices (PMA, PI, Muhlemann, OHI-s). Thus, increased apoptosis in generalized periodontitis against the background of bronchiectasis can act as a factor aggravating the damage to periodontal tissues.

1. Subanova A.A. Features epidemiology and pathogenesis of periodontal diseases (review). Vestnik Kyrgyzsko-Rossiiskogo Slavyanskogo Universiteta. 2015; 15(7): 152–155 (In Russ., English abstract).

2. Tsepov L.M., Mikheeva E.A., Goleva N.A., Nesterova M.M. Chronic generalized periodontitis: the notes to present-days concepts. Parodontologiya. 2010; 15(1) (54): 3–7 (In Russ., English abstract).

3. Zeidán-Chuliá F., Gursoy M., de Oliveira B.H., Gelain D.P., Könönen E., Gursoy U.K., Moreira J.C., Uitto V.J. Focused microarray analysis of apoptosis in periodontitis and its potential pharmacological targeting by carvacrol. Arch. Oral Biol. 2014; 59(5): 461–469. DOI: 10.1016/j.archoralbio.2014.01.007

4. Parakhonskii A.P., Shmal’ko N.M., Tsyganok S.S. Znachenie apoptoza leikotsitov v immunopatogeneze parodontita. Sovremennye Problemy Nauki I Obrazovaniya. 2006; 2: 74–75 (In Russ.).

5. Dabiri D., Halubai S., Layher M., Klausner C., Makhoul H., Lin G.H., Eckert G., Abuhussein H., Kamarajan P., Kapila Y. The role of apoptotic factors in assessing progression of periodontal disease. Int. J. Dent. Oral Sci. 2016; 3(9): 318–325. DOI: 10.19070/2377-8075-1600064

6. Rand J.H., Wu X.X., Lin E.Y., Griffel A., Gialanella P., Mckitrick J.C. Annexin A5 binds to lipopolysaccharide and reduces its endotoxin activity. MBio. 2012; 3(2): pii: e00292-11. DOI: 10.1128/mBio.00292-11

7. Hong M., Park N., Chun Y.J. Role of Annexin A5 on mitochondria-dependent apoptosis induced by tetramethoxystilbene in human breast cancer cells. Biomol Ther (Seoul). 2014; 22(6): 519–524. DOI: 10.4062/biomolther.2014.112

8. Gazhva S.I., Zaplutanova D.A., Eremeev A.F. Problema komorbidnykh zabolevanii v stomatologii. Sovremennye Problemy Nauki I Obrazovaniya. 2015; 6-0: 91 (In Russ.).

9. Sarkisov K.A., Polunina O.S., Bragin E.A., Voronina L.P., Nurzhanova I.V. The condition of basal blood stream in patients with chronic parodontitis on the phone of bronchial asthma. Astrakhanskii Meditsinskii Zhurnal. 2010; 5(3): 100–102 (In Russ., English abstract).

10. Brooks J.K. Psoriasis: A review of systemic comorbidities and dental management considerations. Quintessence Int. 2018; 49(3): 209–217.

11. Ermolaeva L.A., Shishkin A.N., Sheveleva N.A., Pen’kovoi E.A., Sheveleva M.A., Sokolovich N.A., Khabarova O.V., Mikhailova E.S. Features of the periodontal pathology at patients with metabolic syndrome. Uspekhi Gerontologii. 2016; 29(2): 318–323 (In Russ., English abstract).

12. Ní Chróinín D., Montalto A., Jahromi S., Ingham N., Beveridge A., Foltyn P. Oral health status is associated with common medical comorbidities in older hospital inpatients. J. Am. Geriatr. Soc. 2016; 64(8): 1696– 1700. DOI: 10.1111/jgs.14247