EXPERIMENTAL STUDY OF THE ANTIDIARRHOEAL ACTIVITY OF MONTMORILLONITE-CONTAINING ENTEROSORBENT
https://doi.org/10.25207/1608-6228-2017-24-5-82-89
Abstract
Aim. To study the antidiarrhoeal activity of montmorillonite-based enterosorbent on the model of serotonin-induced diarrhea.
Materials and methods. The study was performed on laboratory mice of both genders, acute diarrhea was induced by intraperitoneal injection of serotonin hydrochloride at a dose of 0.32 mg/kg, enterosorbent as an aqueous suspension was administered orally in a wide range of doses 30 minutes prior to serotonin administration. Within 4 hours, the time of diarrhea onset, the number of defecations, the fluid content in the fecal masses was considered. Morphological examination of the small intestine was carried out.
Results. The administration of serotonin led to the development of diarrhea in 100% of animals in 12,8±1,2 minutes, the number of defecations increased 2,5 times compared to intact animals and was 19,5±0,5 times within 4 hours, the fecal masses were predominantly wet and liquid. The use of enterosorbent under the Crim_04 cipher in the maximum dose led to a delay in the development of diarrhea to 73,6±4,1 minutes, a reduction in the number of defecations to 9,4±0,1 and a significant inspissation of stool. The antidiarrhoeal effect of enterosorbent was confirmed morphologically.
Conclusions. Montmorillonite-based enterosorbent under the laboratory cipher Crim_04 has a dose-dependent antidiarrhoeal activity in the modeling of serotonin-induced diarrhea.
About the Authors
A. N. TISHIN
Orel Regional Clinical Hospital
Russian Federation
Russian Federation
Pobedy Boulevard, 10, Orel, Russia, 302028
tel.: 8(919) 436-09-96; Generala Lavrova str., 6, apt. 177,
Zilina, Oryol region, Russia, 302530
M. V. POKROVSKII
Belgorod National Research University
Russian Federation
Pobedy str., 85, Belgorod, Russia, 308015
Russian Federation
Pobedy str., 85, Belgorod, Russia, 308015
O. M. TISHINA
Medical-sanitary Part of the Ministry of Internal Affairs of Russia for Orel region
Russian Federation
Russian Federation
Saltykov-Shchedrin str., 37, Orel, Russia, 302028
V. I. YAKUSHEV
Belgorod National Research University
Russian Federation
Pobedy str., 85, Belgorod, Russia, 308015
Russian Federation
Pobedy str., 85, Belgorod, Russia, 308015
N. V. ZABOLOTSKIH
FSBEI HE KSMU Ministry of Healthcare of Russia
Russian Federation
Russian Federation
Department of Nervous Diseases and Neurosurgery with the Course of Nervous Diseases and Neurosurgery FPC and Faculty
Sedina str., 4 Krasnodar, Russia, 350063
References
1. 10 ведущих причин смерти в мире / Всемир. орг. здравоохранения (ВОЗ). (Информ. бюл.). Женева, 2017. URL: http://www.who.int/mediacentre/factsheets/fs310/ru (дата обращения 02.07.2017). [Top 10 causes of death worldwide. Org. Health (WHO). (Inform.). 2017, Geneva. URL: http://www.who.int/mediacentre/factsheets/fs310/en (circulation date 02.07.2017)].
2. Диарея / Всемир. Орг. Здравоохранения (ВОЗ). (Информационный бюллетень № 330). Женева, 2013. URL: http://www.who.int/mediacentre/factsheets/fs330/ru/ (дата обращения 14.07.2017). [Diarrhea / World. Org. Health (WHO). (Information Bulletin No. 330). 2013. Geneva. URL: http://www.who.int/mediacentre/factsheets/fs330/en/ (circulation date 14.07.2017)].
3. Carretero M.I., Pozo M. Clay and non-clay minerals in the pharmaceutical and cosmetic industries part II. Active ingredients. Appl. Clay Sci. 2010; 47: 171-181.
4. Liashenko N.V. Peculiarities of enterosorption via nasointestinal probe using sorbent diosmectite. Klin. Khir. 2014; 12: 16-18.
5. Юлиш Е. И., Кривущев Б.И. Метод энтеросорбции в лечении синдрома интоксикации. Здоровье ребенка. 2011; 4(31): 76-81. [YUlish E. I., Krivushchev B.I. Metod ehnterosorbcii v lechenii sindroma intoksikacii. Zdorov'e rebenka. 2011; 4 (31): 76-81. (In Russ.)].
6. Тишин А.Н., Бесхмельницына Е.А., Якушев В.И. и др. Противодиарейная активность монтмориллонитсодержащего. Смоленский медицинский альманах. 2017; 1: 347-350. [Tishin A.N., Beskhmel'nicyna E.A., YAkushev V.I., Tishina O.M., Linnik M.S., Krivoruchenko V.B. Protivodiarejnaya aktivnost' montmorillonitsoderzhashchego. Smolenskij medicinskij al'manah. 2017; 1: 347-350. (In Russ.)].
7. Тишин А. Н., Покровский М. В., Тишина О. М. и др. Изучение сорбционной активности энтеросорбента на основе монтмориллонита по отношению к энтеротоксину E.coli на модели изолированных петель кишки. Кубанский научный медицинский вестник. 2017; 24(3): 106-113. DOI: 10.25207 / 1608-6228-2017-24-3-106-113. [Tishin A.N., Pokrovskii M.V., Tishina O.M., Sernov L.N., Provotorov V.YA., Beskhmel'nicyna E.A. Izuchenie sorbcionnoj aktivnosti ehnterosorbenta na osnove montmorillonita po otnosheniyu k ehnterotoksinu E.coli na modeli izolirovannyh petel' kishki. Kubanskij nauchnyj medicinskij vestnik. 2017; 24(3): 106-113. (In Russ., English abstract) DOI: 10.25207 / 1608-6228-2017- 24-3-106-113].
8. Новокшонов А.А., Соколова Н.В. Клиническая эффективность этиопатогенетической терапии энтеросорбентами острых кишечных инфекций у детей. Сonsilium Мedicum. Педиатрия. 2009; 1: 4-8. [Novokshonov A.A., Sokolova N.V. Klinicheskaya ehffektivnost' ehtiopatogeneticheskoj terapii ehnterosorbentami ostryh kishechnyh infekcij u detej. Sonsilium Medicum. Pediatriya. 2009; 1: 4-8. (In Russ.)].
9. Bui Quang Cu , Nguen Hoai Chao, Vesentsev A.I., Buhanov V.D. , Sokolovsky P.V., Mihaylyukova M.O. The antibacterial properties of modified bentonite deposit tam bo. Research result: pharmacology and clinical pharmacology. 2016; 2(3): 63-74. doi: 10.18413/2500-235X -2016-2-3-63-74.
10. Bukhanov V.D., Vezentsev A.I., Filippova O.V., Nadezhdin S.V., Pankova O.N., Firsova T.I., Mikhailyukova V.O., Tishin A.N. The influence of the concentration of montmorillonite containing sorbent and pH of the culture medium on the antibiotic sensitivity of Escherichia coli, as well as the effect of ground on growth of Escherichia. Research result: pharmacology and clinical pharmacology. 2017; 3(1): 97-104. DOI: 10.18413/2500-235X-2017-3-1-97-104.
11. Caihong Hu, Juan Song, Zhaotong You, Zhao Shuang Luan, Weifen Li Zinc oxide- montmorillonite hybrid influences diarrhea, intestinal mucosal integrity, and digestive enzyme activity in weaned pigs. Biological trace element research. 2012; 149: 190-196.
12. Khediri F., Mrad A.I., Azzouz M., Doughi H., Najjar T., Mathiex-Fortunet H., Garnier P., Cortot A. Efficacy of diosmectite (smecta) in the treatment of acute watery diarrhea in adults: a multicentre, randomized, double-blind, placebo-controlled, parallel group study. Gastroenterol Res Pract. 2011; 11: 721-736.
13. Mujawar Q.M., Naganoor R., Ali M.D., Malagi N., Thobbi A.N. Efficacy of dioctahedral smectite in acute watery diarrhea in Indian children: a randomized clinical trial. J Trop Pediatr. 2012; 58: 63-67.
14. Tishin A.N., Krut U.A., Tishina O.M., Beskhmelnitsyna E.A., Yakushev V.I. Physico-chemical properties of montmorillonite clays and their application in clinical practice (review). Research result: pharmacology and clinical pharmacology. 2017; 3(2): 119-128.
15. SMECTA, powder for oral solution in sachets (TRANSPARENCY COMMITTEE, Opinion 24 July 2013). Haute Autorité de Santé. https://www.has-sante.fr/portail/upload/docs/application/pdf/2014-01/smecta_ct12417.pdf. (in French).
16. Бондарев А.В., Жилякова Е.Т. Анализ спроса на адсорбционные кишечные препараты при помощи индекса Вышковского. Фармация и фармакология. 2014; 6(7): 114-116. [Bondarev A.V., ZHilyakova E.T. Analiz sprosa na adsorbcionnye kishechnye preparaty pri pomoshchi indeksa Vyshkovskogo. Farmaciya i farmakologiya. 2014; 6(7): 114- 116. (in Russ.)].
17. Жилякова Е.Т., Самсонов А.А., Голубев Н.Н., Бондарев А.В. Обзор российских энтеросорбционных лекарственных средств. Ремедиум. 2014; 10: 40-47. [ZHilyakova E.T., Samsonov A.A., Golubev N.N., Bondarev A.V. Obzor rossijskih ehnterosorbcionnyh lekarstvennyh sredstv. Remedium. 2014; 10: 40-47. (in Russ.)].
18. Фарма-2020 – экспертная площадка открытого обсуждения [Электронный ресурс]. Стратегия развития фармацевтической промышленности Российской Федерации на период до 2020 г.: URL: http://www.pharma2020.ru/download/1594.htmlpharma2020=0223e3cc41acc42e5/ (дата обращения 21.08.2017). [Farma-2020 – ehkspertnaya ploshchadka otkrytogo obsuzhdeniya [EHlektronnyj resurs]. Strategiya razvitiya farmacevticheskoj promyshlennosti Rossijskoj Federacii na period do 2020 g.: URL: http://www.pharma2020.ru/download/1594.html?pharma2020=0223e3cc41acc42e5/ (data obrashcheniya 21.08.2017). (in Russ.)].
19. Kadowaki M., Nagakura Y., Tomoi M., Mori J., Kohsaka M. Effect of FK1052, a potent 5- hydroxytryptamine 3- and 5-hydroxytryptamine 4 receptor dual antagonist, on colonic function in vivo. Journal of Pharmacology and Experimental Therapeutics. 1993; 266(1): 74-80.
20. Парфенов А.И. Четыре варианта патогенеза и терапии диареи. Терапевтический архив. 2015; 87(12): 5-12. [Parfenov A.I. CHetyre varianta patogeneza i terapii diarei. Terapevticheskij arhiv. 2015; 87(12): 5-12. (in Russ.)].
21. Shcherbakov P., Trubitsyna I., Kirova M. Serotonin and Acetylcholine associated damage to microcirculation in gastric mucosa and cytokine changes in rats. Gastroenterology. 2011; 140: 318-319.
22. Dong Y., Yang C., Wang Z., Qin Z., Cao J., Chen Y. The injury of serotonin on intestinal epithelium cell renewal of weaned diarrhea mice. European Journal of Histochemistry. 2016; 60(2689): 253-261.
23. Tishin A.N., Tishina O.M., Yakushev V.I., Beskhmelnicyna E.A., Linnik M.S., Krivoruchenko V.B. Preclinical study of pharmacological activity of enterosorbente on the basis of montmorillonite. Research result: pharmacology and clinical pharmacology. 2017; 3(3): 37-54.
Review
For citations:
TISHIN A.N., POKROVSKII M.V., TISHINA O.M., YAKUSHEV V.I., ZABOLOTSKIH N.V. EXPERIMENTAL STUDY OF THE ANTIDIARRHOEAL ACTIVITY OF MONTMORILLONITE-CONTAINING ENTEROSORBENT. Kuban Scientific Medical Bulletin. 2017;(5):82-89. (In Russ.) https://doi.org/10.25207/1608-6228-2017-24-5-82-89
Views:
448
Email this article 